Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt | 71119-23-8 | MFCD00065473 | 100g
MES sodium salt | Purity: 99% | MW: 217.22 | 71119-23-8 | MFCD00065473 | 100g
Medchemexpress LLC 4-Morpholineethanesulfonic acid, potassium salt | 39946-25-3 | 99.9% | 10 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. As a Good's buffer, it is utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15 and shows poor affinity with metal ions, making it suitable for studies involving them.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at around pH 6.15
- Poor affinity with metal ions, making it suitable for studies involving metal ions
Medchemexpress LLC MES potassium (2-Morpholinoethanesulphonic acid potassium) | 39946-25-3 | 99.88% | 5 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with pH range 5.5-7.0.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration at varying temperatures.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Exhibits poor affinity with metal ions, making it suitable for studies involving such ions.
Medchemexpress LLC 3-(N-morpholino)propanesulfonic acid sodium salt | 71119-22-7 | 99.8% | 100 G
MOPS sodium salt acts as a buffering agent within biological contexts. This buffer is critical for sustaining the appropriate pH levels in mammalian cell culture media. It also significantly enhances the thermal stability of bovine serum albumin.
- Functions as a buffering agent.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of bovine serum albumin.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES HEPES BIOPERFORMANCE 99.5
NC3239327 HEPES BIOPERFORMANCE 99.5
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 6.8) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Enzo Life Sciences Fixation Permeabilization, (buffer) (100 ml)
Formulation: Liquid. PBS, pH 7.2, containing 4% formaldehyde and 0.1% saponin. Applications: Flow Cytometry. Shipping: Blue Ice Not Frozen. Long Term Storage: +4°C
Teknova 1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 25 g
MES monohydrate | Purity: ≥99.0% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 25 g
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
Boston Bioproducts Inc Tris-EDTA Buffer, TE (20X, pH 8.0) - 250ml
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.
Research Products International Corp RIPA Buffer 2X Solution, 250 ML
Enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins' immunoreactivity and biological activity.
Research Products International Corp TBE, 4 L Cube
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1