Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502472486.JPG-250.jpg)
eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
Medchemexpress LLC AR-13324 analog (mesylate) | 2095432-73-6 | 98.0% | 631.72 | 10 MM * 1 ML
AR-13324 analog mesylate is an analog of AR-13324. AR-13324 is an ROCK and norepinephrine transporter (NET) inhibitor.
- 10 mM * 1 mL in DMSO (ready for reconstitution)
- 10 mM * 1 mL in DMSO
- 5 mg
- 10 mg
- 50 mg
- 100 mg
- 200 mg
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660032.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 50 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent widely used in laboratory applications for pH buffering. This 50g package of MOPS is commonly used for preparing small to medium quantities of buffer solutions for molecular biology, protein chemistry, and electrophoresis. The reagent is effective in maintaining a stable pH range of 6.5 to 7.9, which is essential for stabilizing proteins, nucleic acids, and enzymes during biochemical assays. MOPS is an important component in buffer systems for electrophoresis, chromatography, and enzyme studies where consistent pH is necessary to ensure reliable experimental outcomes.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660241.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Sodium Salt is a biochemical reagent supplied in a 250g package, offering a reliable buffer for experiments requiring precise pH control. This sodium salt form of HEPES maintains a stable pH of 6.8–8.2, which is essential for a variety of biochemical and biological applications such as cell culture, protein analysis, and enzymatic assays. HEPES sodium salt is known for its excellent buffering capacity, ensuring that pH remains constant even during extended incubation periods. It is widely used in research and laboratory environments due to its low toxicity, making it a preferred choice for maintaining stable conditions during biological experiments.
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661848.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 100 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent supplied in a 100g bottle. This buffering agent is commonly used in biochemistry and molecular biology applications. DIPSO is known for its ability to maintain a stable pH in aqueous solutions, particularly at low pH values, making it ideal for use in enzyme assays, protein purification, and other chemical reactions that require precise pH control. It is often employed in applications such as electrophoresis, chromatography, and protein studies. This high-quality reagent is suitable for various laboratory protocols that demand reliable buffering capacity and pH stability.
Biotang Inc PIPES Lysis Buffer with Triton (2X), 125 ml
PIPES Lysis Buffer with Triton (2X), 125 ml.
Medchemexpress LLC 3-morpholino-2-hydroxypropanesulfonic acid | 68399-77-9 | MFCD00009629 | 99.7% | 225.26 g/mol | C7H15NO5S | 500 G
MOPSO is a zwitterionic biological buffer used to maintain stable near-neutral pH in biochemical and cell culture applications. It buffers effectively in the 6.2-7.6 pH range and is supplied as a high-purity powder with supporting analytical documentation for quality control.
- Useful pH range of 6.2-7.6.
- High purity suitable for research applications.
- Appropriate for biological and biochemical buffering needs.
- Supplied as a powder for convenient storage and handling.
- Analytical documentation available (COA, SDS, HNMR, RP-HPLC, MS).
BIOHIPPO Research Grade Anti-Human IL10 Biosimilar Antibody (MK-1966), 100 µg
MK-1966 is a research-grade biosimilar antibody targeting IL10. It reacts with Human. Suitable for ELISA, FACS, Functional assay, Research in vivo. This research-grade biosimilar supports comparability, functional, PK/PD and immunogenicity studies. Supplied as 100 ug.
Alkali Scientific Bicine Ultra Pure, 250 G
Bicine Ultra Pure, available in a 250g package, is a high-quality buffer ideal for a range of biochemical and molecular biology applications. This buffer provides excellent pH stability in the range of 7.6–9.0 and is widely used in protein purification, electrophoresis, and enzyme assays. Its low ionic strength and minimal background interference make it particularly valuable for sensitive applications where precise pH control is necessary. The 250g size is ideal for medium-scale laboratory operations, providing an efficient supply for research projects and experiments requiring reliable buffering capacity over extended periods. Bicine Ultra Pure is a critical reagent in proteomics and genomics research.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 240 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer is a powdered medium formulated for plant tissue culture applications. The 240g powder makes 50 liters of nutrient solution, enriched with MES buffer to stabilize the pH during plant cell culture. The inclusion of MES ensures better pH stability during long incubation periods, especially in environments where pH fluctuations can affect cell growth. This medium is ideal for research in plant biotechnology, as it supports both the growth and differentiation of plant cells, tissues, and organs. It is commonly used for in vitro studies, propagation, and transformation of various plant species.
Medchemexpress LLC 3-(N-morpholino)propanesulfonic acid sodium salt | 71119-22-7 | 99.8% | 100 G
MOPS sodium salt acts as a buffering agent within biological contexts. This buffer is critical for sustaining the appropriate pH levels in mammalian cell culture media. It also significantly enhances the thermal stability of bovine serum albumin.
- Functions as a buffering agent.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of bovine serum albumin.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)